Table 1.
Performance of HPV tests on formalin-fixed paraffin-embedded biopsy material against the ‘reference’ test
| HPV test | Smeets et al. [6] | Schache et al. [7] | Schlecht et al. [12] | ||||||
|---|---|---|---|---|---|---|---|---|---|
| No. | Sensitivity (%) | Specificity (%) | No. | Sensitivity | Specificity | No. | Sensitivity | Specificity | |
| RNA RT-PCR | 48 | 100 | 100 | ND | ND | ND | ND | ND | ND |
| DNA qPCR | 47 | 92 | 97 | ND | ND | ND | ND | ND | ND |
| HPV PCR | 48 | 100 | 89 | ND | ND | ND | ND | ND | ND |
| HPV ISHa | 45 | 83 | 100 | 97 | 88% | 88% | 73 | 67% | 61% |
| p16 IHCb | 44 | 100 | 79 | 97 | 94% | 82% | 80 | 56% | 93% |
| p16 IHC and HPV ISH | 42 | 92 | 100 | 97 | 88% | 90% | ND | ND | ND |
| p16 IHC and HPV PCR | 44 | 100 | 100 | ND | ND | ND | ND | ND | ND |
All studies used RT-PCR on frozen tissue as the ‘reference’ test. HPV16 E6/E7 mRNA [6]. HPV16, 18, 33 E6 mRNA [7]. HPV16 E6 mRNA [12]
aFluorescent HPV16/18 ISH [6]. Chromogenic high-risk HPV ISH (Ventana Medical Systems, USA) [7, 12]
bCINtec Histology (Roche mtm laboratories AG, Germany) [6, 7]. Pharmingen p16 IHC (BD Biosciences, USA) [12]