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. 2012 Jun;14(6):547–558. doi: 10.1596/neo.12230

Figure 1.

Figure 1

SOCS-1 and SOCS-3 are tyrosine phosphorylated in Bcr-Abl expressing cells. (A) 293T cells were cotransfected with Bcr-Abl and SOCS-1, SOCS-2, SOCS-3, SOCS-5, SOCS-6, or SOCS-7 using Lipofectamine. After 48 hours of culture, cells were harvested and cell extracts were then prepared. The SOCS proteins were immunoprecipitated using the His-Tag, and immunoblot analysis was performed using X-press antibody, p-Tyr antibody (4G10), and Abl antibody (AB-2). (B and C) 293T cells were cotransfected with Bcr-Abl and either Flag-tagged SOCS-1 (B) or Flag-tagged SOCS-3 (C). SOCS-1 or SOCS-3 protein was then immunoprecipitated using Flag-tagged protein IP kit (Sigma). Shown are immunoblots probed with anti-SOCS-1 antibody (4H1), anti-SOCS-3 antiserum, and p-Tyr antibody (4G10).