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. 2012 Jun;14(6):547–558. doi: 10.1596/neo.12230

Figure 4.

Figure 4

Bcr-Abl-dependent phosphorylation of SOCS-1 and SOCS-3 impairs their ability to negatively regulate JAK2 activation. (A) 293T cells were cotransfected with JAK2 and empty vector, wild-type, or mutant SOCS-1 in the presence or absence of Bcr-Abl as described in Figure 1. Cell lysates were examined for levels of JAK2, phosphor-JAK2, Bcr-Abl, and SOCS-1 using immunoblot analysis assay. (B and C) Experiments were carried out as described in A. JAK2 was coexpressed with either empty vector, SOCS-3(WT), SOCS-3(Y204F), and SOCS-3(Y221F) (B) or SOCS-3(Y204, 221F) (C) in the presence or absence of Bcr-Abl. Shown are immunoblots probed as indicated.