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. 2012 Jun;14(6):547–558. doi: 10.1596/neo.12230

Figure 5.

Figure 5

Activation of JAK/STAT signaling in K562 cells is attenuated when tyrosine phosphorylation of SOCS-1 or SOCS-3 is disrupted. (A) Shown are immunoblots of K562 cells ectopically expressing SOCS-1(WT) or SOCS-3(WT). Cells were treated with or without imatinib and probed with indicated antibodies. (B) K562 cells ectopically expressing Myc-tagged wild-type or mutant SOCS-1 were lysed and examined for endogenous Bcr-Abl, JAK2, phospho-JAK2 (pJAK2), STAT5, and phospho-STAT5 (pSTAT5). The same lysates were immunoprecipitated with anti-Myc antibody (9E10), and precipitated proteins were then examined for SOCS-1 total protein and pY-SOCS-1 levels. (C) Immunoblot of lysates from K562 cells ectopically expressing Flag-tagged wild-type or mutant SOCS-3 was probed as indicated. The lysates were also immunoprecipitated with anti-Flag antibody and then examined for SOCS-3 and pY-SOCS-3 levels by Western blot analysis.