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. 2012 Jul 11;10:86. doi: 10.1186/1479-5876-10-86

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Copyright ©2012 Chen et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunohistochemical staining of alpha-smooth muscle actin for quantification of small vessel (≤ 15.0 μm) (200x) in ischemic quadriceps at day 14 after CLI induction (n = 9). A to D) Significantly higher number of small vessels (≤ 15 μm) (red arrows) in normal control (C) group than in obesity (OB) and obesity control (OB_C) groups, and notably higher number in OB_C group than in OB group. * vs. other groups with different symbols, p < 0.01. Statistical analysis by ANOVA followed by Bonferroni multiple comparison post hoc test. Scale bars in right lower corner represent 50 μm. HPF = high-power field (200x).