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. Author manuscript; available in PMC: 2012 Jul 11.
Published in final edited form as: J Biol Chem. 1999 Aug 13;274(33):23617–23626. doi: 10.1074/jbc.274.33.23617

Fig. 8. Functional interaction of the GC box and E box of the DOR gene.

Fig. 8

NS20Y cells were transfected with various DOR promoter/luciferase constructs containing either wild type DOR promoter (pD262), or DOR promoter with mutated GC box (pDm226), or DOR promoter with mutated E box (pDm184), or DOR promoter with both mutated GC box and mutated E box (pD262Sp1*/E*). Luciferase activities were normalized to β-galactosidase activity and expressed relative to wild type promoter activity, arbitrarily defined as 100%. Activation was expressed as luciferase activities relative to the luciferase activity of pD262Sp1*/E*, arbitrarily defined as 1. The underlined sequences represent either the GC box or E box, as indicated. Mutant sequences are shown in boldface.