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. Author manuscript; available in PMC: 2012 Jul 11.
Published in final edited form as: J Biol Chem. 1999 Aug 13;274(33):23617–23626. doi: 10.1074/jbc.274.33.23617

Fig. 9. Physical interaction of the GC box and E box of the DOR promoter.

Fig. 9

A, three oligonucleotides were used as probes in EMSA shown in panel B. D232/169 contained the DOR promoter sequence from −232 to −169, with both the GC box and E box (underlined). D232/169E* contained the same sequence as D232/169 except the E box was mutated (in bold). D232/169Sp1* containing the same sequence as D232/169 except the GC box was mutated (in bold). B, EMSA was performed with NS20Y nuclear extracts and indicated probes. Lanes 1, 3, and 6, no competitor; lane 5, probe only; lane 2, in the presence of Sp1 consensus oligonucleotide (as shown in Fig. 3B) as the competitor; lane 4, in the presence of E box consensus (as shown in Fig. 5B) as the competitor; lane 7, in the presence of D198/169 (as shown in Fig. 5B) as the competitor; lane 8, in the presence of the Dm226 and Dm184 (as shown in Fig. 3B and 6A) as the competitors; lane 9, in the presence of the D232/215 (as shown in Fig. 3B) as the competitor.