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. Author manuscript; available in PMC: 2012 Jul 11.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Feb 11;368(3):755–760. doi: 10.1016/j.bbrc.2008.01.164

Fig. 2.

Fig. 2

Dynamic regulation of trimethylated H3K9 on the dor gene. (A) Time-dependent NGF-mediated reversal of trimethylated H3K9. (B) Quantitative real-time PCR analysis of H3K9me3 association. After cells were treated with NGF at the indicated times in the conditioned medium (see Materials and methods), cells were harvested and trimethylated H3K9-bound chromatin DNAs were immunoprecipitated. H3K9me3, trimethylated H3K9; ChIP, chromatin immunoprecipitation; input, DNA before immunoprecipitation. ChIP assays were carried out according to the manufacturer’s protocol as described. Ctl, rabbit serum as a negative control. The ratio of ChIP to input DNA at time zero was normalized to be one unit. Data are means ± SEM of three independent experiments in 3–9 real-time PCR replicates.