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Intracellular distribution of fluorescein isothiocyanate-labeled DNA plasmid/chitosan/polyethylenimine/peptide complexes was observed with a confocal fluorescence microscope in HepG2 cells at 0.5, 2, 4, and 6 hours postincubation.
Notes: The upper panel shows the nucleus (red) and the middle panel shows the fluorescein isothiocyanate plasmid (green). The lower panel shows the overlay of the top two panels.
