Figure 7. c-myc deletion does not affect onset of tumour development.

A. Kaplen–Meier survival graph showing no difference in time to renal tumourigenesis between AhCre⁺ Apc^fl/fl p21^−/− (Apc p21, n = 31, black line) and AhCre⁺ Apc^fl/fl c-Myc^fl/fl p21^−/− mice (Apc c-Myc p21, n = 20, red line) (Log rank p = 0.571), illustrating that c-myc deletion does not affect onset of renal tumourigenesis.

B. H&E showing AhCre⁺ Apc^fl/fl c-Myc^f/fl p21^−/− triple knockout renal tumours.

C. Absence of SA β-gal staining in AhCre⁺ Apc^fl/fl c-Myc^f/fl p21^−/− triple knockout renal tumours.

D. β-catenin IHC in AhCre⁺ Apc^fl/fl c-Myc^f/fl p21^−/− triple knockout renal tumours shows nuclear localization.

E. c-Myc IHC showing upregulation of c-Myc in AhCre⁺ Apc^fl/fl p21^−/− renal tumours.

F. c-Myc IHC shows lack of c-Myc expression in AhCre⁺ Apc^fl/fl c-Myc^f/fl p21^−/− triple knockout renal tumours, showing c-Myc is not required for tumourigenesis. Scale bars (B–F) represent 200 µm.

G and H Strong upregulation of proliferative markers Ki67 (G) and MCM2 (H) showing AhCre⁺ Apc^fl/fl c-Myc^f/fl p21^−/− triple knockout renal tumours deficient tumours are highly proliferative. Scale bars (G and H) represent 20 µm.