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. 2011 Jul;3(7):370–384. doi: 10.1002/emmm.201100145

Figure 6. Effect of inhibition of MMP-1 activity on lung vascular permeability and DIC in septic mice.

Figure 6

A. CF-1 mice (n = 4–5), C57Bl/6 mice (n = 3–4) or Par1−/− C57Bl/6 mice (n = 4) were injected with pure active human MMP-1 (0.1 mg/kg), heat-inactivated human MMP-1 (0.1 mg/kg; inact. MMP-1), TFLLRN peptide (2.5 mg/kg) or PBS vehicle. After 5 min (peptide) or 15 min (MMP-1), Evans blue dye was injected into the internal jugular vein and allowed to circulate for 30 min and the amount of Evans Blue dye that leaked into the lung interstitium was quantified.

B. Lung vascular permeability in CF-1 mice after i.p. injection of LPS (10 mg/kg), and subsequent treatment with subQ MMP Inh-1 (5 mg/kg) or vehicle as measured by Evans Blue dye leakage at the 4 h time point (n = 5).

C. Lung vascular permeability in mice subjected to CLP and injected with vehicle or MMP-Inh-1 immediately after CLP. Permeability was assessed as in (A) at 24 h. p < 0.05 versus vehicle (n = 5).

D-F. CF-1 mice underwent CLP and were injected subQ with vehicle or MMP-Inh-1 (5 mg/kg) immediately after CLP. Blood was drawn by terminal cardiac puncture at 24 or 48 h after the procedure as noted. Platelets were counted in whole blood at 24 h after CLP (D), plasma TAT levels (E) and plasma D-dimer levels (F) were measured by ELISA at 48 h after CLP. (n = 3–9).