Fig. 5.

Differential expression of selected genes in tissue from dark vs. light bars of freshwater fish. qPCR and RNA-seq data are shown for four genes, arranged alphabetically. Light bar expression levels are shown in white bars and dark bar levels in black. For qPCR, sample size is N = 5; error bars represent the standard error. An asterisk indicates significant differences at P < 0.05. For RNA-seq, data from both 21 dph and 35/56 dph samples are shown; sample size is N = 1. RPKM= reads per kilobase per million mapped reads.