Details of primary roots of three genotypes of A. thaliana by cryo-SEM (A–C) and the schemas of their anatomy drawn according to the semi-thin sections (D–F). (A, D) The wild-type Ler possesses a unilayered epidermis consisting of trichoblasts (dark blue) and atrichoblasts (light blue), a unilayered outer cortex (violet), a unilayered endodermis (red) and a vascular cylinder with a unilayered pericycle (yellow) and diarch arrangement of vascular tissues. (B, E) The scr-3 mutant possesses a unilayered epidermis consisting of trichoblasts (dark blue) and atrichoblasts (light blue), an incomplete layer of outer cortex (violet), an incomplete layer of atypical cortex (grey), an incomplete layer of endodermis (red) and a vascular cylinder with a unilayered pericycle (yellow) and diarch arrangement of vascular tissues. (C, F) The mutant shr possesses a unilayered epidermis consisting of trichoblasts (dark blue) and atrichoblasts (light blue), a unilayered atypical cortex (grey) and a vascular cylinder with a unilayered pericycle (yellow) and diarch arrangement of vascular tissues; the endodermis is not developed. ac, atypical cortex (grey); ep, epidermis (blue); oc, outer cortex (violet); en, endodermis (red); pe, pericycle (yellow); x, xylem (brown); ph, phloem (green). Scale bars (A, B, D–F) = 20 µm; (C) = 10 µm.