Fig. 1.

The Yariv reagent. (A) chemical structures of different phenylglycosides including active Yariv (β-d-glucosyl and β-d-galactosyl) and inactive Yariv (α-d-galactosyl and α-d-mannosyl), commercially available at www.biosupplies.com.au. (B) Histochemical staining of arabidopsis root with inactive Yariv. (C) Histochemical staining of arabidopsis root with active Yariv (note the reddish colour of the root). (D) Rocket electrophoresis of AGPs extracted from the roots of wild-type (WT) Arabidopsis thaliana, from the reb1-1 mutant or from the reb1-1 mutant grown in the presence of 10 mm galactose. The numbers at the top of rockets correspond to AGP quantities (in mg). CTR = 0 mg. Note that AGP content is recovered after addition of galactose (Gal) to reb1-1 mutant (0·45 mg). Standard AGPs were extracted and purified from red wine. Scale bars in (B) and (C) = 200 µm.