Table 3.
Overview from several plant species of AGP localization during pollen-grain formation, and in pollen tubes grown in vitro and in vivo
| Species | Probe | Microscopy* | Culture | Cell type† | Labelling pattern† | References |
|---|---|---|---|---|---|---|
| Gymnosperms | ||||||
| Cycadales | ||||||
| Cycas revoluta | JIM13, LM2 | EM/CF | In vitro | PG and PT | JIM13: strong labelling of the intine and PT; LM2: strong labelling PT, no labelling of the intine wall | Yatomi et al. (2002) |
| Ginkgoales | ||||||
| Ginkgo biloba | JIM13, LM2 | EM/CF | In vitro | PG and PT | JIM13 and LM2: strong labelling of the intine and PT wall | Yatomi et al. (2002) |
| Pinales | ||||||
| Podocarpus macrophyllus | JIM13, LM2 | EM/CF | In vitro | PG and PT | JIM13: labelling of the intine and PT wall | Yatomi et al. (2002) |
| Podocarpus nagi | LM2 : weak labelling | |||||
| Abies veitchii | JIM13, LM2 | EM/CF | In vitro | PG and PT | JIM13: labelling of the intine and PT wall | Yatomi et al. (2002) |
| LM2 : no labelling of the PT, labelling of the intine wall | ||||||
| Cedrus deodara | JIM13, LM2 | EM/CF | In vitro | PG and PT | JIM13: strong labelling of the intine and PT wall | Yatomi et al. (2002) |
| Chamaecyparis obtusa | LM2 : no labelling | |||||
| Picea wilsonii | LM2 | FM | In vitro | PT | Whole PT wall | Chen et al. (2008) |
| LM2 | FM | In vitro | De-exined PG | Intine surface | Fang et al. (2008) | |
| Picea meyeri | LM2 | FM | In vitro | PT | Periodic ring-like pattern along the whole PT wall | Chen et al. (2007) |
| Pinus densiflora | JIM13, LM2 | EM/CF | In vitro | PG and PT | JIM13 and LM2: intine wall of PG. Whole PT wall (tip and back) and secretory vesicles | Mogami et al. (1999); Yatomi et al. (2002) |
| LM2: generative cell wall | ||||||
| Pinus bungeana | LM2 | FM | In vitro | De-exined PG | Intine surface | Fang et al. (2008) |
| Pinus banksiana | JIM13, LM2 | EM/CF | In vitro | PG and PT | JIM13and LM2: labelling of the intine and PT wall | Yatomi et al. (2002) |
| Pinus rigida | ||||||
| Cryptomeria japonica | ||||||
| Pinus elliottii | JIM13, LM2 | EM/CF | In vitro | PG and PT | JIM13: labelling of the intine and PT wall | Yatomi et al. (2002) |
| LM2: weak labelling of the PT, no labelling of the intine | ||||||
| Angiosperms | ||||||
| Monocot | ||||||
| Liliales | ||||||
| Lilium longiflorum | JIM13 | FM | In vitro | PG protoplast | Cell surface | Zhao et al. (2004) |
| JIM13 | EM/HPF | In vivo | PT | PM, inner cell wall and secretory vesicles | Roy et al. (1997) | |
| JIM13-16, LM2, MAC207 | FM | In vitro and in vivo | PT | PT tip wall | Jauh and Lord (1996) Mollet et al. (2002) | |
| JIM13, LM2, MAC207 | FM | In vitro and in vivo | Pectinase treated PT | Ring-like pattern along the whole PT wall | Jauh and Lord (1996) | |
| βGlcY (30 µm) | LM | In vitro | PT | PT tip wall, arrest of growth | Mollet et al. (2002) | |
| JIM13, LM2, MAC207 | EM/CF | In vivo | PT | PM, secretory vesicles and generative cell membrane | Jauh and Lord (1996) | |
| Poales | ||||||
| Lolium perenne | JIM13 | EM/CF | In vivo | PG formation | Microspore wall | Wisniewska and Majewska-Sawka (2006) |
| Eudicot | ||||||
| Magnoliales | ||||||
| Annona cherimola | JIM13 | FM | In vitro | PT | Whole PT wall | Mollet et al. (2002) |
| βGlcY (30 µm) | LM | In vitro | PT | Expanded region from the tip, arrest of growth | ||
| Brassicales | ||||||
| Arabidopsis thaliana | LM2, JIM13, | FM | In vitro | PT | LM2 and MAC207: stronger labelling at the tip than the back | Dardelle et al. (2010), Pereira et al. (2005) |
| MAC207 | JIM13: no labelling | |||||
| JIM8, JIM13, | FM | In vivo | PG formation | JIM8 and JIM13: strong labelling of microsporocyte wall, microspore wall, generative membrane and then sperm cells from microspore stage to mature pollen and in the pollen tube | Coimbra et al. (2007) | |
| MAC207, LM2 | MAC207 and LM2: no specific labelling pattern | |||||
| MAC207, JIM8 | EM/PF | In vitro | PG | MAC207: Intine wall close to the PM | van Aelst and van Went (1992) | |
| JIM8: sperm cell membrane | ||||||
| LM2, JIM13, MAC207 | EM/PF | In vivo | PG and PT | PG: intine | Lennon and Lord (2000) | |
| PT: sperm cell membrane | ||||||
| Brassica napus | JIM8 | FM | In vivo | PG formation | Tetrad wall, PM of mature PG, sperm cell membrane during anthesis | Pennel et al. (1991) |
| Caryophyllales | ||||||
| Beta vulgaris | MAC207 | FM | In vitro | PG | PM | Pennel et al. (1989) |
| JIM4, JIM8 | FM | PG formation | In the callose wall of the tetrad | Majewska-Sawka and Rodriguez-Garcia (2006) | ||
| JIM4: no labelling | ||||||
| JIM13: strong gold labelling in the callose wall of the tetrad | ||||||
| JIM13, LM2 | EM/CF | JIM8 and LM2: weak gold labelling in the callose wall of the tetrad | ||||
| Solanales | ||||||
| Nicotiana tabacum | PCBC3, MAC207, JIM8, JIM13 | EM/CF | In vitro | PG and PT | PCBC3: PG: PM | Fergusson et al. (1999) |
| PT: discontinuous labelling at the PM along the PT, between the inner and outer cell wall layers, in secretory vesicles and callose plugs | ||||||
| MAC207, JIM8, JIM13 : no labelling | ||||||
| MAC207 | FM | In vitro | PT | No labelling | Li et al. (1992) | |
| Cellulase treated PT | No tip labelling, strong periodic ring-like pattern back from the tip | |||||
| Pectinase treated PT | No tip labelling, weak periodic ring-like pattern back from the tip | |||||
| MAC207, JIM8 | EM/CF | In vitro | PG and PT | PG: uniform in the intine | Li et al. (1995) | |
| PT: discontinuous labelling along the outer wall of the PT. Labelling in the secretory vesicles and generative cell membrane. No tip labelling | ||||||
| βGlcY (30 µm) | LM | In vitro | PT | No labelling, no arrest of PT growth | Mollet et al. (2002) | |
| JIM4, JIM13, LM2, | FM | In vitro | PG formation and PT | JIM13: microspore wall and whole PT | Qin et al. (2007) | |
| JIM4 and LM2: weak labelling | ||||||
| βGlcY (50–100 µm) | LM | In vitro | PT growth | Slow down of PT growth | ||
| JIM13 | EM/CF | In vivo | PG and PT | PG: intine wall and vesicles | ||
| PT: cell wall, generative and sperm cell membranes | ||||||
| Lycopersicum peruvianum | PCBC3 | EM/CF | In vitro | PG and PT | PG: PM and generative cell membrane | Fergusson et al. (1999) |
| PT: between inner and outer cell wall layers. No labelling at the tip | ||||||
| Ericales | ||||||
| Actinidia deliciosa | JIM13 | FM | In vitro | PT | Whole PT with periodic brighter ring-like pattern along the PT and at the tip | Speranza et al. (2009) |
| JIM13, JIM8 | EM/CF | In vitro | PT | JIM8, 13 and 14: cytoplasm, PM and the outer cell wall | ||
| JIM14, MAC207 | JIM13: tube tip wall | |||||
| MAC207: no labelling | ||||||
* EM, Electron microscopy; FM, fluorescence microscopy; LM, light microscopy; CF, chemical fixation of the sample; PF, plunge freezing fixation; HPF, high-pressure freezing fixation.
† PT, Pollen tube, PG, pollen grain; PM, plasma membrane.