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. Author manuscript; available in PMC: 2012 Jul 11.
Published in final edited form as: Eur J Immunol. 2009 Nov;39(11):2973–2980. doi: 10.1002/eji.200939712

Figure 2. Intranuclear IL-1α is retained by necrotic microglia.

Figure 2

BV-2 microglia were seeded at low, medium or high density (1, 5 and 10 × 105 cells/mL respectively), LPS-treated (1μg/mL, 3 hours) and either kept in normoxic conditions or killed by 24 hours OGD. % total LDH (A) and IL-1α (B) release into media were quantified. Data are from n = 6 independent experiments. ***p<0.001 One-way ANOVA with post-hoc Bonferroni multiple comparison test. Remaining adherent cells in normoxia- (C) and OGD-treated (D) cultures of low density (Ci, Di) and high density BV-2 cells (Cii, Dii) were IL-1α-immunostained (green) with PI (red) and DAPI (blue) co-staining. Images are from one of n = 3 independent experiments. Scale bar = 40 μm.