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. Author manuscript; available in PMC: 2013 May 21.
Published in final edited form as: Biotechnol Prog. 2012 May 21;28(3):846–855. doi: 10.1002/btpr.1542

Figure 2.

Figure 2

Western blot of HC-related proteins in lysates from transient transfectants expressing mAb 277 Ala and Gly variants.

HC-related proteins were probed with an HRP-conjugated α-human Fcγ F(ab')2. Top panel: Non-reduced NEM-treated transient lysates 24 h after transfection. Middle panel: Reduced transient lysates 24 h after transfection. Bottom Panel: Non-reduced transient lysates 72 h after transfection. The bands corresponding to HC (50 kDa), HCLC (75 kDa), HC2 (100 kDa), HC2LC (125 kDa), and HC2LC2 (150 kDa) are labeled.