Table 3.
Intracellular versus extracellular protein levels in the highest expressing Ala and Gly stable cell lines
| Construct | Total Protein/Cell (pg/cell) | IgG/Cell (pg/cell)a | % IgG/Cell | Supernatant IgG (μg/mL)b |
|---|---|---|---|---|
| Ala-138 | 263.2 | 7.1 | 2.7% | 181.0 ± 4.6 |
| Gly-26 | 293.1 | 0.6 | 0.2% | 3.8 ± 0.07 |
|
| ||||
| Ala/Gly | 0.9 | 11.8 | 13.5 | 47.6 |
Error is ±1 standard deviation of biological replicates (n=3)
a
Value is corrected for background binding onto the Protein A Biosensors and was subtracted before determining the % intracellular IgG per cell.
b
IgG titer in supernatant on day of sampling (day 4)