Figure 7. Additive effects of SMS B3 and SMSB4.

Mannan was immobilized on microtiter plates and allowed to activate 4% NHS containing 5 µg/ml of either serpin (110 nM SMSB3 or 93 nM SMSB4) alone or mixed together. As a negative control BSA was used at a concentration of 10 µg/ml. After 20 min of incubation the plates were washed and the deposited C9 was detected with specific antibodies. An average of three independent experiments is presented with bars indicating means ± SEM. *p<0.05, ***p<0.001 by t test (GraphPad Prism).