Figure 1. Staphylococcus aureus prevents osteoblast growth and proliferation over a 21 day period.
Osteoblasts (5×105 cells/ml) were preincubated with either control buffer (——) or formaldehyde fixed S. aureus Newman (-----), Newman Δspa (⋅⋅⋅⋅⋅), or Newman spa (pCU1spa+) (— ⋅ —). (A) Following days 7, 14 and 21 osteoblasts were removed by trypsinization and proliferation was determined by counting cells on a haemocytometer. (B) Osteoblasts were also visualised using an inverted bright field microscope (Leica, DMIL) at x200 magnification., *P<0.0001, n = 5.