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Osteoblasts (1Ć106 cells/well) were incubated with either control buffer, S. aureus Newman, Newman Īspa or Newman spa (pCU1spa +) for 24 hrs. Media from uninfected and infected osteoblasts was removed and centrifuged at x 10,000g for 2 minutes. Soluble RANKL was detected using an ELISA kit. *P<0.01, **P<0.001, nā=ā3
