Figure 5. Staphylococcus aureus inhibits osteoclastogenesis.

Pre-osteoclasts were seeded (2×10⁴ cells/well) in 12-well tissue culture plates and cultured for 21 days. Conditioned media from osteoblasts (4 days), with either control buffer (——) or formaldehyde fixed S. aureus Newman (-----), Newman Δspa (⋅⋅⋅⋅⋅), or Newman spa (pCU1spa ⁺) (— ⋅ —) was then transferred to the pre-osteoclasts. Osteoclastogenesis was determined by measuring tartarate resistant alkaline phosphatase (TRAP). *P<0.05, **P<0.001 n = 5.