Table 2. Ten-point standardized rating scale for EAE clinical score monitoring.

	Score	Description
Tail	0	Tail erected
	1	Extremity of the tail flaccid with a tendency to fall
	2	Tail completely flaccid and drags on the table
Hind limbs	0	Energetic walk and doesn’t drag his paws
	1	Either one of the following test is positive: (i) flip test: a delay suggests hind-limb weakness, (ii) on the wire cage top: one or both limbs frequently slip between the bars, sign of partial paralysis
	2	Both previous tests are positive
	3	One or both hind limbs show signs of paralysis but some movement is preserved
	4	Both hind legs are paralyzed and the mouse drags them when moving
Fore limbs	0	Front paws active for grasping and walking and head erected
	1	Walking is difficult due to a weakness in one or both of the paws, another sign is head drooping
	2	One forelimb is paralyzed, head lost much of its muscle tone
	3	Cannot move, and food and water are unattainable
Bladder	0	Full control of bladder
	1	Mouse incontinent

The final score for each animal is determined by the addition of all the above mentioned categories. Note: Score  =  15 for dead animal.