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. 2012 Jul 11;7(7):e40500. doi: 10.1371/journal.pone.0040500

Figure 1. Systemic bacterial load is not influenced by Wolbachia infection.

Figure 1

Least squares means for bacterial load (±1SE) of five Wolbachia-infected lines [WOLB(+)TET(-)] and genetically matched lines that have been cured of Wolbachia [WOLB(+)TET(+)], as well as five Wolbachia-uninfected lines [WOLB(-)TET(-)] and genetically paired tetracycline treated lines[WOLB(-)TET(+)]. Note that the WOLB category on the x-axis refers to initial Wolbachia-infection status prior to antibiotic treatment, rather than infection status at the time of experimental infections. Bacterial load was measured 24 hours after infection with the pathogenic bacteria (A) P. rettgeri (B) L. monocytogenes and (C) S. typhimurium. Assays were performed 2, 4, and 6 generations after ending tetracycline treatment, with three replicates in each generation. For each replicate, bacterial load was measured in 3 pools of 5 flies from every line.