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. 2012 Jul 11;7(7):e40500. doi: 10.1371/journal.pone.0040500

Table 1. Description of Factors Tested in Analyses of Variance.

Factor Effect Type Effect Measured
line(WOLB) fixed effect of each genetic line nested within the factor WOLB
WOLB fixed Wolbachia status of each line prior to tetracycline treatment
TET fixed whether or not flies were treated with tetracycline
GEN fixed number of generations since tetracycline treatment (2, 4, or 6)
REP(GEN) random replicate day on which the experiment was performed
WOLB*TET fixed differential effects of tetracycline on flies with and without Wolbachia
TET*GEN fixed differential effects of tetracycline across the generations tested
GEN*line(WOLB) fixed differential effects of line across the generations tested
TET*line(WOLB) fixed differential effects of tetracycline on flies of each line
WOLB*TET*GEN fixed different effects of tetracycline on flies with and without Wolbachia and across generations
TET*GEN*line(WOLB) fixed differential effects of tetracycline on flies of each line and across generations