Figure 1. NO-dependent effects of leptin on glycerol (A) and FA (B) release from rat WAT explants.

Explants were pre-treated or not with L-NAME (1 mmol/L) for 30 min, then exposed or not to either leptin (10 µg/L), SNAP (1 mmol/L) or IFN-γ (50 µg/L) for 2 h in KRB medium containing 2% BSA (lipolysis medium). Results are expressed as the percent of glycerol or FA relative to the corresponding untreated control. Crude values for control were 4.57±0.13 nmol.mg⁻¹ tissue.2 h⁻¹ for glycerol and 4.60±0.47 nmol.mg⁻¹ tissue. 2 h⁻¹ for FA. Each value represents the mean ± SEM, (n = 4) *, P<0.01 vs. control; ^a P<0.01 vs. leptin-treated explants.