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. 2012 Jul 11;7(7):e40614. doi: 10.1371/journal.pone.0040614

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Johnson et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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, Alexa-488 labeled TSP-1 binds to the surface of paraformaldehyde fixed T. cruzi trypomastigotes as seen by fluorescence microscopy. A’, DAPI stained preparation of A showing parasite nuclear DNA (arrow heads) and k-DNA (arrows). B, Binding of Alexa-488 labeled TSP-1 to paraformaldehyde fixed T. cruzi trypomastigotes is inhibited by 100X unlabeled TSP-1. B’, DAPI stained preparation of B showing parasite nuclear DNA (arrow heads) and k-DNA (arrows). This figure shows the results of a representative experiment selected from three independent experiments with similar results.