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. 2012 Jul 11;7(7):e40614. doi: 10.1371/journal.pone.0040614

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Johnson et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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, Alexa labeled TcCRT IgG binds to the surface of T. cruzi trypomastigotes as revealed by flow cytometry. Shift in the peak of fluorescence indicates that parasite surface is stained with labeled TcCRT IgG. B, Fluorescence intensity of parasites stained with labeled isotype control (left panel) and anti-TcCRT IgG (right panel) showing the high percentage (99.2%) of stained parasites. This figure shows the results of a representative experiment selected from three independent experiments with similar results.