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. 2012 Jul 11;7(7):e40618. doi: 10.1371/journal.pone.0040618

Figure 6. ITC binding assay ofΔN10IPO (monomeric IPO) with methylated carbohydrates.

Figure 6

The thermal changes were detected by 0.5 mM ΔN10IPO with 12.5 mM Me-Man (A), 0.75 mM ΔN10IPO with 20 mM Me-Glc (B) and 1 mM ΔN10IPO with 25 mM Me-Gal (C). The upper panel of figures was presented by 18 injections and 2 µl/per injection. The interval of injection time is 180 sec. The 18 experimental data were almost fitted for a 1∶1 binding model (one-site of fitting) with Microcal Origin 7.0 software (the bottom panel). In each bottom panel, X-axis indicates the molar ratio of protein-carbohydrate and Y-axis indicates the thermal change in each injection. In the titration of ΔN10IPO with Me-Gal, no obvious thermal changes could be detected.