Table 2. Thermodynamics values of IPO and ΔN10IPO titrated with various carbohydrates*.
| ProteinCarbohydrates | c value | na | K A(103 M−1) | ΔH(kcal/mol) | ΔG(kcal/mol) | -TΔS(kcal/mol) |
| IPO | ||||||
| Me-Man | 7.04 | 1.0 | 7.04(±0.20) | −5.56(±0.28) | −5.17(±0.15) | 0.39(±0.31) |
| Me-Glc | 2.01 | 1.0 | 2.01(±0.05) | −4.12(±0.11) | −4.57(±0.12) | −0.45(±0.02) |
| Me-Gal | 4.09 | 1.0 | 4.09(±0.06) | −5.14(±0.13) | −4.94(±0.02) | 0.20(±0.12) |
| Man | 0.32 | 1.0 | 0.11(±0.00) | −4.61(±0.14) | −2.75(±0.08) | 1.86(±0.06) |
| Glc | 0.10 | 1.0 | 0.03(±0.00) | −3.17(±0.10) | −2.04(±0.06) | 1.13(±0.03) |
| Gal | 0.17 | 1.0 | 0.06(±0.00) | −4.78(±0.15) | −2.39(±0.07) | 2.39(±0.07) |
| ΔN10IPO | ||||||
| Me-Man | 18.97 | 1.0 | 37.94(±1.18) | −13.64(±0.33) | −5.99(±0.72) | 7.66(±0.75) |
| Me-Glc | 6.80 | 1.0 | 13.60(±0.12) | −9.53(±0.25) | −5.57(±0.01) | 3.97(±0.10) |
| Me-Gal | ND | – | – | – | – | – |
*
Triple repeats were analyzed and the values represented the average with standard errors in parenthesis.
a
The n value was fixed at 1.0 for fitting the curves.
ND represents not determined.