Table 1. Quantitative FRET of 2-Color SERCA Constructs.

FRET Efficiency (%)
	510	577	662	C-term
Intact cells	15.9±0.2	16.8±0.3	7.0±0.3	6.5±0.2
+ Ca	20.6±0.3	18.0±0.2	7.2±0.1	8.1±0.2
+ TG	6.6±0.4	13.7±0.3	9.4±0.2	9.5±0.3
+ EGTA	5.3±0.1	8.3±0.1	4.5±0.2	5.8±0.6
Probe Separation Distance (Å)
	510	577 *	662 *	C-term
Intact cells	65.7±0.2	65.0±0.2	76.6±0.6	77.7±0.4
+ Ca	62.4±0.2	64.1±0.1	76.3±0.2	74.7±0.3
+ TG	77.5±0.8	67.7±0.3	72.6±0.3	72.5±0.5
+ EGTA	80.5±0.3	74.3±0.2	82.9±0.6	79.3±1.5

^*Incomplete fluorophore maturation for 576 and 661 may yield underestimated distance.