Table 5. Gain of potential congruence in L158E mutant of PBP-A.
| a | b | c | d | e | ||||||||||||||
| 1E25 | Ser70/OG | Lys73/NZ | Ser130/OG | Lys234/NZ | Glu166/OE1 | |||||||||||||
| 2J7V | Ser61/OG | LYS64/NZ | Ser122/OG | LYS219/NZ | Leu158/CD2 | |||||||||||||
| 2J9O | Ser61/OG | Lys64/NZ | Ser122/OG | Lys219/NZ | Glu158/OE1 | |||||||||||||
| 1NZO | Ser44/OG | Lys47/NZ | Ser110/OG | Lys213/NZ | Leu153/CD2 | |||||||||||||
| ab | ac | ad | ae | bc | bd | be | cd | ce | de | |||||||||
| 1E25 | PD | −125.6 | 22.4 | −189.1 | 310.7 | 148.1 | −63.5 | 436.3 | −211.5 | 288.2 | 499.7 | |||||||
| 1E25 | D | 2.8 | 3.2 | 4.7 | 5.5 | 3.6 | 5.6 | 5 | 2.9 | 8 | 10 | |||||||
| 2J7V | PD | −134.5 | 38.7 | −135.6 | −112.4 | 173.3 | −1 | 22.1 | −174.3 | −151.1 | 23.2 | |||||||
| 2J7V | D | 3 | 3 | 4.4 | 7.1 | 3.1 | 5.4 | 5.8 | 2.9 | 8.7 | 10.1 | |||||||
| 2J9O | PD | −162.3 | −16.4 | −198.3 | 247.7 | 146 | −36 | 410 | −181.9 | 264 | 445.9 | |||||||
| 2J9O | D | 2.7 | 3 | 4.5 | 4.5 | 3 | 5.3 | 4 | 2.8 | 6.7 | 8.6 | |||||||
| 1NZO | PD | −241.6 | −68.8 | −277.9 | −188.2 | 172.8 | −36.2 | 53.5 | −209.1 | −119.4 | 89.7 | |||||||
| 1NZO | D | 3.1 | 4.2 | 6.3 | 6.5 | 5.1 | 6.7 | 5.5 | 2.7 | 10 | 12.1 | |||||||
Pairwise potential difference (PD) and distance (D) between cognate pairs in a Class A Blase (PDBid:1E25), a PBP-5 protein (PDBid:1NZO), the wildtype PBP-A (PDBid:2J7V) and the L158E PBP-A mutant (PDBid:2J9O). The L158E PBP-A mutant has gains potential congruence with the Class A Blase, as compared to the wildtype PBP-A. Distances are in A. See Methods section for units of potential.