FIG. 1.

(A) Domain diagram of recombinant proteins hlFVII-LDP, hlFVII-LDP-mCherry, and mCherry. His tag, purifying tag composed of six histidines; hlFVII, the light chain of human factor VII; Linker, a flexible linker composed of triplicate GGGGS. LDP, the apoprotein of lidamycin; mCherry, a monomeric red fluorescent protein. (B) Purified recombinant proteins hlFVII-LDP (lane 2), hlFVII-LDP-mCherry (lane 3), and mCherry (lane 4) were analyzed by SDS-PAGE. (C) Schematic presentations of the dissociation of LDM into AE and LDP and the reconstitution of hlFVII-LDP-AE. LDM, lidamycin; AE, active enediyne; SDS-PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis.