Fig. 3.

The BITC-mediated inhibition of breast cancer cell migration is not affected by co-treatment with a γ-secretase inhibitor. a Immunoblotting for cleaved Notch1 and cleaved Notch2 proteins using lysates from the MDA-MB-231 cells and the SUM159 cells treated for 24 hours with DMSO (control) or 5 μM BITC in the absence or presence of 20 μM DAPT. Number above band represents change in protein level relative to DMSO-treated control. b Representative images (Boyden chamber assay) depicting migration by the MDA-MB-231 cells and the SUM159 cells after 24-hour treatment with DMSO or 5 μM BITC in the absence or presence of 20 μM DAPT (100× magnification). c Quantitation of cell migration after 24-hour treatment with DMSO or 5 μM BITC in the absence or presence of 20 μM DAPT. Results shown are mean ± SD (n = 3). *Significantly different (P<0.05) between the indicated groups by one-way ANOVA followed by Bonferroni’s multiple comparison test. Each experiment was repeated twice and representative data from one such experiment are shown.