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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: J Neurosci Res. 2012 Apr 14;90(9):1701–1712. doi: 10.1002/jnr.23055

Figure 1. Dicer1 is downregulated in differentiated oligodendrocyte cells.

Figure 1

A. Western blot analysis of Dicer1 expression in 10-day differentiated and undifferentiated Oli-neu cells (n=2). hnRNPL serves as control for loading accuracy of nuclear proteins, GAPDH for cytoplasmic proteins and α-tubulin for both. Dicer1 band detected in the cytoplasm was measured by densitometry and corrected by the GAPDH. The value is indicated below each lane. B. Western blot analysis of PLP/DM20 and CNPase expression in 10-day differentiated and undifferentiated Oli-neu cells (n=2). PLP/DM20 and CNPase are used as control for differentiation of Oli-neu cells and β-tubulin as loading control. C. Real-time RT-PCR analysis of Dicer1 expression in undifferentiated and 10-day differentiated Oli-neu cells. The expression of Dicer1 in differentiated Oli-neu cells is expressed as percent of that in undifferentiated cells, which is set as 1 (n=4). ** p<0.01 (Student’s t test). D. Real-time RT-PCR analysis of Dicer1 expression in EGFP+ OL. The expression of Dicer1 at P21 is expressed as percent of that at P 1 which is set as 1 (n=4). ** p<0.01 (Student’s t test).