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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: J Neurosci Res. 2012 Apr 14;90(9):1701–1712. doi: 10.1002/jnr.23055

Figure 4. Mouse PLP 3′ UTR contains regulatory elements that control PLP gene expression.

Figure 4

A. Schematics of the luciferase-PLP 3′UTR containing constructs. The UTRPLP-1.6 and UTRPLP-0.6 were generated by cloning the 1.6 kb and 0.6 kb (GenBank Accession #NM_011123) sequences downstream of the PLP stop codon in the vector psiCHECK™-2 (Promega), respectively. The UTRPLP-2.2 contains the 0.6 kb sequences of the mouse PLP 3′UTR downstream of the 1.6 fragment, which are uniquely present in the 3.2 kb PLP mRNA (GenBank Accession #AK134554). Dashed line represents deleted portion from the 3.2 kb transcript 3′UTR. B, C and D. Bar graphs showing the luciferase activity derived from the constructs shown in A. Luciferase activity was assayed 48 hrs (B) and 72 hrs (C and D) after transfection into Oli-neu cells. The data are expressed as ratio of the Renilla luciferase to the firefly luciferase activity (R/F), and the R/F of the empty vector is set as 1. Error bars represent SD. n≥3. ** P<0.01 (Student’s t test); ns, not significant.