Fig. 4.

Enhancement of survivin expression by ILF3/NF110 overexpression. a, promoter activities were examined using various lengths of the survivin gene promoter region in HEK-293 cells. Luciferase activity was measured in the presence of ILF3–1a (white) or control plasmid (black). The firefly luciferase enzyme activity was normalized to Renilla luciferase enzyme activity. The values are the means ± S.D. from three independent experiments. b, promoter activities of D3 containing −109 to +16 bp relative to the transcription start site were examined at different concentrations of YM155 in the presence of ILF3–1a or control plasmid in HEK-293 cells. c, the promoter activities of D3 (−109 to +16 bp) were examined in the presence of ILF3–1a, -2a, or -3a or control plasmid in HEK-293 cells. Comparable protein expression levels following overexpression were confirmed by Western blotting (supplemental Fig. 4).