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SILAC-based quantitative proteomic method for revealing the 5-Aza-CdR-induced perturbation of protein expression in the global proteome. A, Flowcharts of forward SILAC combined with LC-MS/MS for the comparative analysis of protein expression in Jurkat-T cells upon 5-Aza-CdR treatment. B, The distribution of expression ratios (treated/untreated) for the quantified proteins, including those quantified in only one set of SILAC labeling experiment.
