Fig. 3.

Mass spectra of tryptic plasma peptides enriched using the anti-EGYR TXP antibodies without sucrose density gradient separation (A), in the top 300-μl fraction (B), in the middle 400-μl fraction (C), and in the bottom 500-μl fraction following sucrose density gradient ultracentrifugation (D). The samples containing antibodies conjugated with the dye Cy5 were added to each ultracentrifuge run to visualize the location of antibodies within the sucrose layers after centrifugation (insets). The peptides corresponding to nontargeted high abundant plasma proteins are marked with asterisks. a.u., arbitrary units.