Table I. Comparison of Surface Accessibility to Percent Reactivity for wheat eIF4E reacted with NN at a 1:15 protein/NN molar ratio. Surface accessibility was calculated using GetArea. Percent Reactivity was calculated using equation 1. “NA” indicates a peptide that was not found in either its modified or unmodified form. Standard deviations ranged from 10–20% for all percent reactivities.

Residue	Solvent accessibility %			% Reactivity
	Dimer	Mutant	Monomer	Tryptic	GluC	ArgC
Ala 1	46	65	41	12	11	5
Lys 18	43	34	96	19	5	25
Lys 52	76	74	50	0	0	0
Lys 63	5	9	20	0	NA	0
Lys 65	70	100	94	32	NA	11
Lys 69	100	20	48	0	0	0
Lys 80	27	20	20	0	NA	0
Lys 89	77	80	67	NA	NA	NA
Lys 122	40	38	92	79	5	70
Lys 131	31	26	31	0	0	0
Lys 144	68	73	66	1	2	0
Lys 147	22	15	13	0	0	0
Lys 153	42	42	84	0	0	0
Lys 165	80	43	50	0	0	0
Lys 169	40	81	91	60	9	34
Lys 172	28	94	72	0	0	0