Table I. O-glycosylation sites identified in the present study (See supplemental Figs.). Site assignments are given for the Uniprot entry corresponding to the best characterized bovine sequence. Uniprot IDs in parantheses refer to the protein identified by database search. Names with asterisk refer to proteins described as “uncharacterized protein”, the name of the closest well characterized (human) homolog is given. Legend for modification sites: new; discovered by us earlier (14, 30); known confirmed now; known found earlier; known in human found now, known in human found earlier. Reference data for known sites is given in supplemental File S2.

Uniprot ID	Protein name	Modification site	Enrichment protocol
A2I7N2	SERPINA3–6	31-gT	Both
A4IFA5	VASN protein	455-gS, 460-gT	Both
A5D7R6	ITIH2 protein	673-gS, 691-gT	Both
A5PK77	SERPINA11 protein	387-gT	mIEX
A5PKA3	CCDC80 protein	89-gT	ERLIC
A6QLD8	ADAMTSL4 protein	605-gT	Both
E1BB91	Collagen alpha-3(VI) chain*	2929-gT	ERLIC
E1BCW0	Hepatocyte growth factor activator*	355-gT, 360-gT, 365-gT	Both
E1BI67	Interleukin-18-binding protein*	50-gS	ERLIC
E1BKQ9	Polypeptide N-acetylgalactosaminyltransferase 5*	429-gT	Both
F1MER7	Basement membrane-specific heparan sulfate proteoglycan core protein*	3374-gT	ERLIC
F1MMK9	Protein AMBP*	198-gT	Both
F1N1I6	Gelsolin*	27-gT, 34-gT, 44-gT	Both
O18977 (F1MPK6)	Tenascin-X	3146-gT or 3147-gT(682-gT or 683-gT)	ERLIC
P00735	Prothrombin	193-gT, 205-gT, 206-gS	ERLIC
P00743	Coagulation factor X	485-gT	ERLIC
P00744	Vitamin K-dependent protein Z	388-gT	Both
P01030	Complement C4 (fragments)	420-gT	Both
P01044 (F1MNV4)	Kininogen-1	136-gT, 149-gS or 150-gT, 399-gT, 400-gT, 406-gS, 581-gS, 586-gT, 605-gT	Both
P02672	Fibrinogen alpha chain	464-gT, 525-gT	ERLIC
P02676	Fibrinogen beta chain	4-gT	Both
P06868	Plasminogen	366-gT, 378-gT	Both
P07224	Vitamin K-dependent protein S	104-gT	ERLIC
P07456 (B8QGI3)	Insulin-like growth factor 2	99-gT, 106-gT, 154-gS, 163-gT, 168-gT, 173-gS; 174-gS	Both
P07589	Fibronectin	280-gT, 2156-gT, (2157-gT)	Both
P12763	Alpha-2-HS-glycoprotein	217-gT, 271-gS, 280-gT, 282-gS, 296-gS, 314-gT, 320-gS, 324-gS, 325-gS, 334-gT, 341-gS	Both
P17690	Beta-2-glycoprotein 1	32-gS, 33-gT	mIEX
P19035	Apolipoprotein C-III	90-gS or 92-gT	ERLIC
P28800	Alpha-2-antiplasmin	24-gS/27-gS/28-gT, 398-gT, 400-gT, 489-gS	24/27/28 mIEX; 398&400 ERLIC 489 both
P50448	Factor XIIa inhibitor	74-gT	mIEX
P81187	Complement factor B	26-gT	ERLIC
P81644	Apolipoprotein A-II	40-gT	Both
Q03247	Apolipoprotein E	31-gT, 32-gT, 211-gT, 307-gS, 309-gT, 310-gS	Both
Q05717	Insulin-like growth factor-binding protein 5	171-gT	ERLIC
Q0VCM5	Inter-alpha-trypsin inhibitor heavy chain H1	643-gS, 648-gT	Both
Q28083	Collagen alpha-1(XI) chain (Fragment)	86-gT	ERLIC
Q28107	Coagulation factor V	1151-gS, 1154-gT, 1171-gT	Both
Q29RQ1	Complement component C7	696-gT	mIEX
Q2KIU3	Protein HP-25 homolog 2	72-gT	Both
Q32KM8	Augurin	47-gT	ERLIC
Q32PI4 (F1N4M7)	Complement factor I	57-gT	mIEX
Q3MHN2	Complement component C9	24-gT, 26-gS	ERLIC(mIEX)
Q3SWW8	Thrombospondin-4	270-gT, 282-gS, 284-gT	ERLIC
Q3SYR5	Apolipoprotein C-IV	35-gT	ERLIC
Q3T052 Q5EA67	Inter-alpha-trypsin inhibitor heavy chain H4	629-gS, 635-gS, 677-gS, 683-gS, 686-gS, 688-gT, 689-gS, 695-gS, 698-gT, 705-gT, 706-gT, 708-gT, (719-gS)	Both
Q3ZBS7	Vitronectin	63-gT, 97-gT, 98-gT, 107-gT, 142-gT or 143-gS	Both
Q58CQ9	Pantetheinase	504-gT	Both
Q58D34	Peptidase inhibitor 16	408-gT	ERLIC
Q58D62	Fetuin-B	19-gT, 20-gS, 157-gS, 173-gT, 262-gS, 273-gT, 292-gT, 295-gT, 299-gT	Both
Q95121	Pigment epithelium-derived factor	34-gT	Both
Q9N2I2	Plasma serine protease inhibitor	35-gT, 36-gT	Both