Figure 2.

Demethylation reactivates expression of CACNA2D3. (A) MDA-MB-453 and MDA-MB-436 cells were grown in the presence of AZA, trichostatin A (TSA) or both. Expression of CACNA2D3 was determined by qPCR. (B) AZA-dependent demethylation of the CACNA2D3 CpG island correlates with re-expression of CACNA2D3. Cells were treated with AZA and TSA as above. CpG methylation was determined by pyrosequencing and the level of methylation is represented by the intensity of shading in the circles, each of which represents an individual CpG dinucleotide in the amplified fragment.