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. 2012 Jun 7;107(2):352–359. doi: 10.1038/bjc.2012.251

Figure 3.

Figure 3

miR-21, miR-17, and miR-19a promoted the proliferation and migration of LoVo cells and directly targeted PTEN. (A and G) LoVo-VC cells overexpressing miR-21, miR-17, and miR-19a exhibited significantly enhanced proliferative capability, as determined by a CCK8 assay, and (B and H) an enhanced invasive ability, as determined by a Transwell assay. (D, E, J and K) However, miR-21, miR-17 and miR-19a inhibitors antagonised the proliferation and migration of LoVo-PRL-3 cells. (C and I) The PTEN protein, a known target of miR-21 and miR-17–92, was significantly downregulated by miR-21 and miR-19a mimics in LoVo-VC cells. (F and L) miR-21 and miR-19a inhibitors restored the expression of the PTEN protein in LoVo-PRL-3 cells. (O) Overexpressed PTEN in LoVo-PRL-3 cells. (M and N) PTEN antagonised the phenotype exerted by these miRNAs in proliferation and invasion assay in LoVo-PRL-3 cells (*P<0.01, **P<0.01; Student’ t-test; n=3).