Figure 2.

VE-821 inhibits the growth of a 3D tumour model. Spheroids were grown from MG-U87 cells to average size of 400 μm and grown for further 9 days. (A) Spheroids were treated with either DMSO or 1 μℳ VE-821 and were replaced every 2 days for 9 days. The graph represents the average volume of at least 12 spheroids throughout treatment. (B) Before fixing, spheroids were treated with EF5. Spheroids were then fixed, sectioned and stained for EF5, demonstrating the presence of hypoxic regions within the spheroids. EF5 regular stain – ELK5-A8 antibody, EF5-competed stain – ELK5-A8+EF5. Significance values: *P<0.05.