Figure 2.

Effects of heat and radiofrequency plasma glow discharge (RFGD) pretreatments on (A) type I collagen (α1), (B) parathyroid hormon-related peptide (PTH-rP), and (C) alkaline phosphatase mRNA expression in MC3T3 osteoprogenitor cells cultured on Ti6Al4V disks. The levels of type I collagen (α1), PTH-rP, and alkaline phosphatase mRNA expression measured by quantitative real time polymerase chain reaction for treated disks at the indicated points in time are presented as a percentage of the levels of mRNA expression measured for untreated disks. Data represent means±standard error for 3 to 8 independent cultures at each point in time. FN: fibronectin. ^a-c)Significantly greater (P<0.05, 0.01, 0.001) than untreated disks. ^d-f)Significantly greater (P<0.05, 0.01, 0.001) than RFGD-pretreated disks based on analysis of variance.