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. 2012 Jun 30;42(3):95–104. doi: 10.5051/jpis.2012.42.3.95

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Copyright © 2012 Korean Academy of Periodontology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/).

PMC Copyright notice

Effects of heat and radiofrequency plasma glow discharge (RFGD) pretreatments on (A) osteopontin, (B) bone sialoprotein, and (C) osteocalcin mRNA expression in MC3T3 osteoprogenitor cells cultured on Ti6Al4V disks. The levels of osteopontin, bone sialoprotein, and osteocalcin mRNA expression measured by quantitative real time polymerase chain reaction for treated disks at the indicated points in time are presented as a percentage of the levels of mRNA expression measured for untreated disks. Data represent means±standard error for 3 to 9 independent cultures at each point in time. FN: fibronectin. ^a)Significantly greater (P<0.05) than untreated disks. ^b)Significantly greater (P<0.05) than heat-pretreated disks based on analysis of variance.