Figure 2.
Modulation of claudin-1 expression induces ZEB-1 expression and inhibits E-cadherin expression. (A) Semiquantitative reverse-transcription polymerase chain reaction. (B) Immunoblot analysis. Total RNA or protein lysates from SW480control, SW480claudin-1, SW620control, and SW260siRNA cells were used. (C and D) Densitometric analysis. Bars represent mean ± standard deviation (n = 3). (E) Immunofluorescent staining to determine cellular localization of claudin-1, E-cadherin, and ZEB-1. 4=,6-Diamidino-2-phenylindole (DAPI) served as nuclear marker.