Skip to main content
. Author manuscript; available in PMC: 2012 Jul 12.
Published in final edited form as: Am J Med Sci. 2012 Jul;344(1):41–51. doi: 10.1097/MAJ.0b013e318234c132

FIGURE 8.

FIGURE 8

Aging is associated with increase in number of Thy-1–negative fibroblasts in the lung, and this is not influenced by DNA methylation. Lungs were harvested from uninjured 3-month-old and 24-month-old C57BL/6 mice, and primary fibroblasts were isolated for analysis of Thy-1. (A and B) Phase contrast microscopy of passage 3 young (A) and old (B) primary lung fibroblasts in culture. Magnification, 20×. (C) Quantitative PCR to assess Thy-1 expression in lung fibroblasts isolated from young (open bars) and old (closed bar) mice and in lung fibroblasts from old mice treated with 5′AZA to induce DNA demethylation (gray bar). P < 0.001 as analyzed by 1-way analysis of variance. (D) Representative histogram of flow cytometry for Thy-1 performed in young (gray line) and old (black line) lungs. (E and F) Percent (E) and mean (F) fluorescent intensity of Thy-1 expressed by lung fibroblasts isolated from young and old lungs as analyzed by flow cytometry. *P < 0.05 compared with young group by t test and post-test analysis.