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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: J Neurochem. 2011 Sep 20;119(2):354–363. doi: 10.1111/j.1471-4159.2011.07364.x

Fig. 4.

Fig. 4

Interaction of N-cadherin with C99 and the effect of N-cadherin expression on C99 dimerization. (a) NcadHA and C99-Flag were co-expressed in HEK293 cells. Cell lysates were pulled down with anti-Flag antibody, followed by the blotting with anti-HA antibody. (b) HEK293 cells were co-transfected C99-Flag and C99-myc in the presence or absence of NcadHA. Cell lysates were pulled down with anti-Flag antibody, followed by the blotting with anti-myc antibody. (c) The ratio of C99 dimer/Total C99 was compared between the cells transfected with control vector and NcadHA. (n.s.: p = 0.4858, n = 3). (d) HEK293 cells were co-transfected with C99luc1 and C99luc2 in the presence or absence of NcadHA. Luciferase activity in NcadHA + C99luc1 + C99luc2-transfected cells was not significantly different from that in C99luc1 + C99luc2-transfected cells. (luc1 + luc2 n = 6, C99luc1 + luc2 n = 6, luc1 + C99luc2 n = 6, C99luc1 + C99luc2 n = 6, NcadHA + C99luc1 + C99luc2 n = 6).