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. 2012 Apr 24;31(13):2937–2951. doi: 10.1038/emboj.2012.109

Figure 5.

Figure 5

Activation of L-type VGCCs increases insertion of GABAA receptors. (A) Insertion of pHBBSβ3WT containing GABAA receptors over time. Newly inserted pHBBSβ3WT is labelled red (Aexa-594-Bgt). Graph represents the ratio of newly inserted pHBBSβ3 to total pHluorin fluorescence intensity over time. (B) Nucleofected hippocampal neurons expressing pHBBSβ3WT were incubated with Alexa-594-Bgt with or without Bay K 8644 (scale bar 10 μm). Graph shows quantification of newly inserted pHBBSβ3WT with Bay K 8644 expressed as the surface/total pHBBSβ3WT fluorescence intensity ratio normalized to control (Ctrl) values (data are plotted as mean±s.e. ***P<0.001; n>20; three independent cultures; t-test). (C) Hippocampal neurons were treated with Bay K 8644. Lysates were immunoblotted with anti-phosphorylated β3S408/9 IgGs (p-S408/9) and anti-p-S383 IgGs. Graph represents quantification of the ratio of band intensities of P-S408/9 to total β3 normalized to control (Ctrl) values (NS: not significantly different; n=4; t-test). (D) Hippocampal neurons were treated with myristoylated dynamin inhibitory peptide prior to incubation with or without Bay K 8644. Immunoblots show total and cell surface levels of β3 subunits as indicated. Graph represents quantification of the ratio of band intensities of cell surface and total β3 subunits, normalized to control (Ctrl) values (*P<0.05, n=3, t-test).